|Application ||WB, IHC, E|
|Other Accession||NP_002711, 5531, 171366 (rat)|
|Calculated MW||35080 Da|
|Other Names||Serine/threonine-protein phosphatase 4 catalytic subunit, PP4C, Pp4, 220.127.116.11, Protein phosphatase X, PP-X, PPP4C, PPP4, PPX|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-PPP4C Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C- PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.|
|Cellular Location||Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination. Lee DH, et al. Nat Struct Mol Biol, 2010 Mar. PMID 20154705.
Protein phosphatase 4 regulates apoptosis in leukemic and primary human T-cells. Mourtada-Maarabouni M, et al. Leuk Res, 2009 Nov. PMID 19539371.
Association and mutation analyses of 16p11.2 autism candidate genes. Kumar RA, et al. PLoS One, 2009. PMID 19242545.
PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint. Nakada S, et al. EMBO Rep, 2008 Oct. PMID 18758438.
PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4. Chen GI, et al. J Biol Chem, 2008 Oct 24. PMID 18715871.
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