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Goat Anti-PRPF31 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-PRPF31 Antibody AF1870a
    AF1870a (1 µg/ml) staining of HeLa cell lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WWY3
Other Accession NP_056444, 26121, 68988 (mouse)
Reactivity Human
Predicted Mouse, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 55456 Da
Additional Information
Gene ID 26121
Other Names U4/U6 small nuclear ribonucleoprotein Prp31, Pre-mRNA-processing factor 31, Serologically defined breast cancer antigen NY-BR-99, U4/U6 snRNP 61 kDa protein, Protein 61K, hPrp31, PRPF31, PRP31
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-PRPF31 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRPF31
Synonyms PRP31
Function Involved in pre-mRNA splicing. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
Cellular Location Nucleus speckle. Nucleus, Cajal body. Note=Predominantly found in speckles and in Cajal bodies
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.


Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation. Schneider M, et al. Nat Struct Mol Biol, 2010 Feb. PMID 20118938.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
Study of gene-targeted mouse models of splicing factor gene Prpf31 implicated in human autosomal dominant retinitis pigmentosa (RP). Bujakowska K, et al. Invest Ophthalmol Vis Sci, 2009 Dec. PMID 19578015.
Novel PRPF31 and PRPH2 mutations and co-occurrence of PRPF31 and RHO mutations in Chinese patients with retinitis pigmentosa. Lim KP, et al. Arch Ophthalmol, 2009 Jun. PMID 19506198.
PRPF31 alternative splicing and expression in human retina. Tanackovic G, et al. Ophthalmic Genet, 2009 Jun. PMID 19373678.

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$ 325.00
Cat# AF1870a
(40 western blots)
Availability: 7-10 days
Bulk Size
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