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Goat Anti-SSP29 / ANP32B Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-SSP29 / ANP32B Antibody AF2035a
    AF2035a (0.1ug/ml) staining of Human Tonsil lysate (35 µg protein in RIPA buffer). Primary incubated was 1 hour. Detected by chemiluminescence.
  • IHC - Goat Anti-SSP29 / ANP32B Antibody AF2035a
    AF2035a (3.8 µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92688
Other Accession NP_006392, 10541, 67628 (mouse), 170724 (rat)
Reactivity Human
Predicted Mouse, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 28788 Da
Additional Information
Gene ID 10541
Other Names Acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, Putative HLA-DR-associated protein I-2, PHAPI2, Silver-stainable protein SSP29, ANP32B, APRIL, PHAPI2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-SSP29 / ANP32B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANP32B
Synonyms APRIL, PHAPI2
Function Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.
Cellular Location Isoform 1: Nucleus. Note=Accumulates in the nuclei at the S phase.
Tissue Location Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.
Research Areas
Citations (0)

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Solution structure of histone chaperone ANP32B: interaction with core histones H3-H4 through its acidic concave domain. Tochio N, et al. J Mol Biol, 2010 Aug 6. PMID 20538007.
Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells. Shen SM, et al. Carcinogenesis, 2010 Mar. PMID 20015864.
Phosphorylation of the HuR ligand APRIL by casein kinase 2 regulates CD83 expression. Chemnitz J, et al. Eur J Immunol, 2009 Jan. PMID 19130553.
Promoter region-specific histone incorporation by the novel histone chaperone ANP32B and DNA-binding factor KLF5. Munemasa Y, et al. Mol Cell Biol, 2008 Feb. PMID 18039846.
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Jeronimo C, et al. Mol Cell, 2007 Jul 20. PMID 17643375.

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$ 325.00
Cat# AF2035a
(40 western blots)
Availability: 7-10 days
Bulk Size
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