|Application ||WB, E|
|Other Accession||NP_055060, 9616, 19823 (mouse)|
|Calculated MW||12683 Da|
|Other Names||RING-box protein 2, Rbx2, CKII beta-binding protein 1, CKBBP1, RING finger protein 7, Regulator of cullins 2, Sensitive to apoptosis gene protein, RNF7, RBX2, ROC2, SAG|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-RNF7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||RBX2, ROC2, SAG|
|Function||Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (PubMed:10851089). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity). Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Validation of SAG/RBX2/ROC2 E3 ubiquitin ligase as an anticancer and radiosensitizing target. Jia L, et al. Clin Cancer Res, 2010 Feb 1. PMID 20103673.
E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Huang DT, et al. Mol Cell, 2009 Feb 27. PMID 19250909.
Regulation of heat shock-induced apoptosis by sensitive to apoptosis gene protein. Lee SJ, et al. Free Radic Biol Med, 2008 Jul 15. PMID 18454945.
SAG/ROC2/RBX2 is a HIF-1 target gene that promotes HIF-1 alpha ubiquitination and degradation. Tan M, et al. Oncogene, 2008 Feb 28. PMID 17828303.
SAG/ROC-SCF beta-TrCP E3 ubiquitin ligase promotes pro-caspase-3 degradation as a mechanism of apoptosis protection. Tan M, et al. Neoplasia, 2006 Dec. PMID 17217622.
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