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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Goat Antibodies   >   UBE2F/NEDD8-conjugating enzyme Antibody (C-Term)   

UBE2F/NEDD8-conjugating enzyme Antibody (C-Term)

Peptide-affinity purified goat antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q969M7
Other Accession NP_542409.1, 140739
Predicted Human, Mouse, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 21077 Da
Additional Information
Gene ID 140739
Other Names NEDD8-conjugating enzyme UBE2F, 6.3.2.-, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, Ubiquitin-conjugating enzyme E2 F, UBE2F, NCE2
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE2F/NEDD8-conjugating enzyme Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2F
Synonyms NCE2
Function Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Tissue Location Widely expressed (at protein level).
Research Areas
Citations (0)

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This antibody is expected to recognise isoform 1 (NP_542409.1) only.


A Nedd8 conjugation pathway is essential for proteolytic targeting of p27Kip1 by ubiquitination. Podust VN, Brownell JE, Gladysheva TB, Luo RS, Wang C, Coggins MB, Pierce JW, Lightcap ES, Chau V. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4579-84. PMID: 10781063

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$ 325.00
Cat# AF2305a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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