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MYH9 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - MYH9  Antibody (internal region) AF2869a
    AF2869a (0.3 µg/ml) staining of Human peripheral blood lymphocytes lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • WB - MYH9  Antibody (internal region) AF2869a
    AF2869a (1 µg/ml) staining of NIH3T3 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35579
Other Accession NP_002464.1, 4627
Reactivity Human, Mouse
Predicted Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 226532 Da
Additional Information
Gene ID 4627
Other Names Myosin-9, Cellular myosin heavy chain, type A, Myosin heavy chain 9, Myosin heavy chain, non-muscle IIa, Non-muscle myosin heavy chain A, NMMHC-A, Non-muscle myosin heavy chain IIa, NMMHC II-a, NMMHC-IIA, MYH9
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYH9 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYH9
Function Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Colocalizes with actin filaments at lamellipodia margins and at the leading edge of migrating cells.
Tissue Location In the kidney, expressed in the glomeruli. Also expressed in leukocytes.
Research Areas
Citations (0)

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Nonmuscle myosin heavy chain IIA mediates integrin LFA-1 de-adhesion during T lymphocyte migration Morin NA, Oakes PW, Hyun YM, Lee D, Chin YE, King MR, Springer TA, Shimaoka M, Tang JX, Reichner JS, Kim M J Exp Med. 2008 Jan 21;205(1):195-205. Epub 2008 Jan 14. PMID: 18195072

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$ 325.00
Cat# AF2869a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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