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VRK2 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - VRK2 Antibody (internal region) AF2995a
    AF2995a (2 µg/ml) staining of K562 nuclear lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q86Y07
Other Accession NP_001129499.1, NP_006287.2, NP_001123954.1, NP_001123955.1, 7444
Reactivity Human
Predicted Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 58141 Da
Additional Information
Gene ID 7444
Other Names Serine/threonine-protein kinase VRK2,, Vaccinia-related kinase 2, VRK2
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVRK2 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VRK2
Function Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.
Cellular Location Isoform 1: Cytoplasm. Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Mitochondrion membrane; Single-pass type IV membrane protein
Tissue Location Isoform 1 and isoform 2 are expressed in various tumor cell lines. Expression of isoform 1 inversely correlates with ERBB2 in breast carcinomas (at protein level) Widely expressed. Highly expressed in fetal liver, skeletal muscle, pancreas, heart, peripheral blood leukocytes and testis
Research Areas
Citations (0)

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This antibody is expected to recognize all isoforms (NP_001123952.1, NP_001123954.1, NP_001123955.1, NP_001129499.1). Reported variants represent identical protein: NP_001123953.1, NP_006287.2.


Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities. Sanz-García M, López-Sánchez I, Lazo PA. Molecular & cellular proteomics : MCP 2008 Nov 7 (11): 2199-214. PMID: 18617507

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$ 325.00
Cat# AF2995a
(40 western blots)
Availability: 7-10 days
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