|Application ||WB, E|
|Other Accession||NP_001129499.1, NP_006287.2, NP_001123954.1, NP_001123955.1, 7444|
|Calculated MW||58141 Da|
|Other Names||Serine/threonine-protein kinase VRK2, 22.214.171.124, Vaccinia-related kinase 2, VRK2|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||VRK2 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.|
|Cellular Location||Isoform 1: Cytoplasm. Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Mitochondrion membrane; Single-pass type IV membrane protein|
|Tissue Location||Isoform 1 and isoform 2 are expressed in various tumor cell lines. Expression of isoform 1 inversely correlates with ERBB2 in breast carcinomas (at protein level) Widely expressed. Highly expressed in fetal liver, skeletal muscle, pancreas, heart, peripheral blood leukocytes and testis|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize all isoforms (NP_001123952.1, NP_001123954.1, NP_001123955.1, NP_001129499.1). Reported variants represent identical protein: NP_001123953.1, NP_006287.2.
Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities. Sanz-García M, López-Sánchez I, Lazo PA. Molecular & cellular proteomics : MCP 2008 Nov 7 (11): 2199-214. PMID: 18617507
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