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ACAT1 (aa257-269) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - ACAT1 (aa257-269) Antibody (internal region) AF3063a
    AF3063a (0.01 µg/ml) staining of Human Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P24752
Other Accession NP_000010.1, 38
Reactivity Human, Mouse, Rat
Predicted Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 45200 Da
Additional Information
Gene ID 38
Other Names Acetyl-CoA acetyltransferase, mitochondrial, 2.3.1.9, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACAT1 (aa257-269) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACAT1
Synonyms ACAT, MAT
Function This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (PubMed:1715688, PubMed:7728148, PubMed:9744475). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (PubMed:1715688, PubMed:7728148, PubMed:9744475). The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA (PubMed:17371050). Thereby, it plays a major role in ketone body metabolism (PubMed:17371050, PubMed:1715688, PubMed:7728148, PubMed:9744475).
Cellular Location Mitochondrion.
Research Areas
Citations (0)
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References

Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A, American journal of physiology. Endocrinology and metabolism 2009 Aug 297 (2): E474-82. PMID: 19625677

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$ 341.00
Cat# AF3063a
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