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ANGPT1 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - ANGPT1 Antibody (internal region) AF3302a
    HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human ANGPT1 with DYKDDDDK tag probed with AF3302a(1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AF3302a (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15389
Other Accession NP_001137.2, 284, 11600 (mouse), 89807 (rat)
Reactivity Human
Predicted Mouse, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 57513 Da
Additional Information
Gene ID 284
Other Names Angiopoietin-1, ANG-1, ANGPT1, KIAA0003
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANGPT1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA0003
Function Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Cellular Location Secreted.
Research Areas
Citations (0)

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An autocrine linkage between matrix metalloproteinase-14 and Tie-2 via ectodomain shedding modulates angiopoietin-1-dependent function in endothelial cells. Onimaru M, Yonemitsu Y, Suzuki H, Fujii T, Sueishi K, Arteriosclerosis, thrombosis, and vascular biology 2010 Apr 30 (4): 818-26. PMID: 20056911

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$ 325.00
Cat# AF3302a
(40 western blots)
Availability: 7-10 days
Bulk Size
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