|Other Accession||NP_057336.3, 57448, 12211 (mouse), 313876 (rat)|
|Predicted||Human, Mouse, Rat, Cow|
|Calculated MW||530255 Da|
|Other Names||Baculoviral IAP repeat-containing protein 6, 6.3.2.-, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE, Ubiquitin-conjugating BIR domain enzyme apollon, APOLLON, BIRC6, KIAA1289|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BIRC6 / Apollon (aa 4724 -4735) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification.|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane. Midbody. Endosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Concentrates in a pericentriolar compartment in interphase, moves partially to spindle poles in metaphase, and finally localizes to the spindle midzone and the midbody in telophase and during cytokinesis|
|Tissue Location||Expressed in brain cancer cells.|
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Provided below are standard protocols that you may find useful for product applications.
Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. Lopergolo A, Pennati M, Gandellini P, Orlotti NI, Poma P, Daidone MG, Folini M, Zaffaroni N, British journal of cancer 2009 Mar 100 (5): 739-46. PMID: 19223905
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