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NOX1 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - NOX1 Antibody (internal region) AF3415a
    AF3415a (0.1µg/ml) staining of lysate of cell line colorectal adenocarcinoma (A) and HeLa (B) (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y5S8
Other Accession NP_008983.2, NP_39249.1, 27035, 237038 (mouse)
Reactivity Human, Mouse
Predicted Cow, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 64871 Da
Additional Information
Gene ID 27035
Other Names NADPH oxidase 1, NOX-1, 1.-.-.-, Mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, NOX1, MOX1, NOH1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNOX1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOX1
Synonyms MOX1, NOH1
Function NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor.
Cellular Location Cell projection, invadopodium membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Tissue Location [Isoform NOH-1L]: Detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize both reported isoforms (NP_008983.2; NP_39249.1).

References

Transcriptional regulation of NADPH oxidase isoforms, Nox1 and Nox4, by nuclear factor-kappaB in human aortic smooth muscle cells. Manea A, Tanase LI, Raicu M, Simionescu M, Biochemical and biophysical research communications 2010 Jun 396 (4): 901-7. PMID: 20457132

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$ 341.00
Cat# AF3415a
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