|Application ||WB, E|
|Other Accession||NP_542409.1, 140739, 67921 (mouse), 363284 (rat)|
|Predicted||Human, Mouse, Pig, Dog, Cow|
|Calculated MW||21077 Da|
|Other Names||NEDD8-conjugating enzyme UBE2F, 6.3.2.-, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, Ubiquitin-conjugating enzyme E2 F, UBE2F, NCE2|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NEDD8 conjugating enzyme (N Terminus) Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.|
|Tissue Location||Widely expressed (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA. Mol Cell. 2009 Feb 27;33(4):483-95. PMID: 19250909
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