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NEDD8 conjugating enzyme (N Terminus) Antibody (N-Term)

Peptide-affinity purified goat antibody

  • WB - NEDD8 conjugating enzyme (N Terminus) Antibody (N-Term) AF3518a
    AF3518a (0.05 µg/ml) staining of Rat Pancreas lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q969M7
Other Accession NP_542409.1, 140739, 67921 (mouse), 363284 (rat)
Reactivity Rat
Predicted Human, Mouse, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 21077 Da
Additional Information
Gene ID 140739
Other Names NEDD8-conjugating enzyme UBE2F, 6.3.2.-, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, Ubiquitin-conjugating enzyme E2 F, UBE2F, NCE2
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEDD8 conjugating enzyme (N Terminus) Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2F
Synonyms NCE2
Function Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Tissue Location Widely expressed (at protein level).
Research Areas
Citations (0)

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E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA. Mol Cell. 2009 Feb 27;33(4):483-95. PMID: 19250909

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$ 325.00
Cat# AF3518a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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