|Application ||WB, E|
|Other Accession||NP_001672.1, NP_733765.1, 488, 11938 (mouse), 29693 (rat)|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||114757 Da|
|Other Names||Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, 126.96.36.199, Calcium pump 2, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A2, ATP2B|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SERCA2 / ATP2A2 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein|
|Tissue Location||Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).
Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells. Satoh K, Matsu-Ura T, Enomoto M, Nakamura H, Michikawa T, Mikoshiba K. J Biol Chem. 2011 Jun 10;286(23):20591-9. PMID: 21515674
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