|Application ||WB, E, EIA|
|Other Accession||NP_002583.1, 5111, 18538 (mouse), 25737 (rat)|
|Reactivity||Human, Mouse, Rat|
|Predicted||Pig, Dog, Cow|
|Calculated MW||28769 Da|
|Other Names||Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PCNA (aa111-122) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.|
|Cellular Location||Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.|
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Reported variants represent identical protein: NP_872590.1, NP_002583.1
Dysregulation of DNA polymerase ? recruitment to replication forks results in genomic instability. Jones MJ, Colnaghi L, Huang TT. EMBO J. 2011 Dec 13. PMID: 22157819
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