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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Goat Antibodies   >   MK5 / MAPKAPK5 (aa192-206) Antibody (internal region)   

MK5 / MAPKAPK5 (aa192-206) Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - MK5 / MAPKAPK5 (aa192-206) Antibody (internal region) AF3924a
    HEK293 lysate (10ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with AF3923a (0.5ug/ml) in right panel and with AF3924a (0.5ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.
  • IP - MK5 / MAPKAPK5 (aa192-206) Antibody (internal region) AF3924a
    AF3923a and AF3924a (1.5ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with mouse anti-MK5 / PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).
  • IHC - MK5 / MAPKAPK5 (aa192-206) Antibody (internal region) AF3924a
    AF3923a (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8IW41
Other Accession NP_003659.2, NP_620777.1, 8550, 17165 (mouse), 498183 (rat)
Reactivity Human, Mouse
Predicted Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 54220 Da
Additional Information
Gene ID 8550
Other Names MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAP-K5, MAPKAPK-5, MK-5, MK5,, p38-regulated/activated protein kinase, PRAK, MAPKAPK5, PRAK
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMK5 / MAPKAPK5 (aa192-206) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PRAK
Function Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras- induced senescence and phosphorylating p53/TP53. Involved in post- transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
Cellular Location Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export
Tissue Location Expressed ubiquitously.
Research Areas
Citations (0)

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A novel function of p38-regulated/activated kinase in endothelial cell migration and tumor angiogenesis. Yoshizuka N, Chen RM, Xu Z, Liao R, Hong L, Hu WY, Yu G, Han J, Chen L, Sun P. Mol Cell Biol. 2012 Feb;32(3):606-18. PMID: 22124154

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$ 325.00
Cat# AF3924a
(40 western blots)
Availability: 7-10 days
Bulk Size
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