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BCAR1 Antibody (internal region)

Peptide-affinity purified goat antibody

  • IHC - BCAR1 Antibody (internal region) AF4000a
    AF4000a (5 µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • WB - BCAR1 Antibody (internal region) AF4000a
    AF4000a (1 µg/ml) staining of MCF7 (A) and Human Breast cancer (B) lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P56945
Other Accession NP_001164185.1, NP_001164186.1, NP_001164187.1, NP_001164188.1, NP_001164189.1, NP_055382.2, NP_001164190.1, NP_001164191.1, NP_001164192.1, 9564, 12927 (mouse), 25414 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 93372 Da
Additional Information
Gene ID 9564
Other Names Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, Cas scaffolding protein family member 1, p130cas, BCAR1, CAS, CASS1, CRKAS
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCAR1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCAR1
Synonyms CAS, CASS1, CRKAS
Function Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
Cellular Location Cell junction, focal adhesion. Cytoplasm. Note=Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
Tissue Location Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line
Research Areas
Citations (0)

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This antibody is expected to recognize reported isoforms1, 2, 3, 5, 6 and 7.


BCAR1 protein plays important roles in carcinogenesis and predicts poor prognosis in non-small-cell lung cancer. Huang W, Deng B, Wang RW, Tan QY, He Y, Jiang YG, Zhou JH. PloS one 2012 7 (4): e36124. PMID: 22558353

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$ 325.00
Cat# AF4000a
(40 western blots)
Availability: 7-10 days
Bulk Size
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