|Application ||WB, E|
|Other Accession||NP_006200.3, NP_001035181.1, NP_001124335.1, 5168|
|Calculated MW||98994 Da|
|Other Names||Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, 220.127.116.11, Autotaxin, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ENPP2 / AUTOTAXIN (aa698-712) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931). Major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:27754931). Also can act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:15769751, PubMed:12176993). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed:1733949). May have a role in induction of parturition (PubMed:12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility- stimulating factor (PubMed:1733949, PubMed:11559573).|
|Tissue Location||Detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize iall reported isoforms (NP_006200.3; NP_001035181.1, NP_001124335.1).
ATX and LPA receptor 3 are coordinately up-regulated in lipopolysaccharide-stimulated THP-1 cells through PKR and SPK1-mediated pathways. Li S, Xiong C, Zhang J. FEBS Lett. 2012 Mar 23;586(6):792-7. PMID: 22314276
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