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Transcription factor E2F1 (aa314-327) Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - Transcription factor E2F1 (aa314-327) Antibody (internal region) AF4033a
    AF4033a (1 µg/ml) staining of HeLa nuclear lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01094
Other Accession NP_005216.1, 1869
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 46920 Da
Additional Information
Gene ID 1869
Other Names Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1, E2F1, RBBP3
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTranscription factor E2F1 (aa314-327) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name E2F1
Synonyms RBBP3
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812).
Cellular Location Nucleus.
Research Areas
Citations (0)

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c-Myc and E2F1 drive PBK/TOPK expression in high-grade malignant lymphomas. Hu F, Gartenhaus RB, Zhao XF, Fang HB, Minkove S, Poss DE, Rapoport AP. Leukemia research 2013 Apr 37 (4): 447-54. PMID: 23237560

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$ 325.00
Cat# AF4033a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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