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Transcription factor E2F4 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - Transcription factor E2F4 Antibody (internal region) AF4034a
    AF4034a (0.3 µg/ml) staining of A43 (A) and HeLa (B) lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16254
Other Accession NP_001941.2, 1874, 104394 (mouse), 100360427 (rat)
Reactivity Human
Predicted Mouse, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 43960 Da
Additional Information
Gene ID 1874
Other Names Transcription factor E2F4, E2F-4, E2F4
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTranscription factor E2F4 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name E2F4
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Cellular Location Nucleus.
Tissue Location Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)

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Coordinated repression of cell cycle genes by KDM5A and E2F4 during differentiation. Beshiri ML, Holmes KB, Richter WF, Hess S, Islam AB, Yan Q, Plante L, Litovchick L, Gévry N, Lopez-Bigas N, Kaelin WG, Benevolenskaya EV. Proceedings of the National Academy of Sciences of the United States of America 2012 Nov 109 (45): 18499-504. PMID: 23093672

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$ 325.00
Cat# AF4034a
(40 western blots)
Availability: 7-10 days
Bulk Size
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