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EDF1 Antibody (internal region)

Peptide-affinity purified goat antibody

  • IHC - EDF1 Antibody (internal region) AF4035a
    AF4035a (5 µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • WB - EDF1 Antibody (internal region) AF4035a
    AF4035a (0.3 µg/ml) staining of A549 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60869
Other Accession NP_003783.1, NP_694880.1, 8721, 59022 (mouse), 678956 (rat)
Reactivity Human
Predicted Mouse, Rat, Pig
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 16369 Da
Additional Information
Gene ID 8721
Other Names Endothelial differentiation-related factor 1, EDF-1, Multiprotein-bridging factor 1, MBF1, EDF1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEDF1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EDF1
Function Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
Cellular Location Cytoplasm. Nucleus. Note=Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin
Tissue Location Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues
Research Areas
Citations (0)

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This antibody is expected to recognize both reported isoforms (NP_003783.1; NP_694880.1).


Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism. Brendel C, Gelman L, Auwerx J. Molecular endocrinology (Baltimore, Md.) 2002 Jun 16 (6): 1367-77. PMID: 12040021

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$ 325.00
Cat# AF4035a
(40 western blots)
Availability: 7-10 days
Bulk Size
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