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NR5A2 / LRH1 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - NR5A2 / LRH1 Antibody (internal region) AF4048a
    AF4048a (1 µg/ml) staining of HepG2 nuclear lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00482
Other Accession NP_995582.1, NP_003813.1, 2494, 26424 (mouse), 60349 (rat)
Reactivity Human
Predicted Mouse, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 61331 Da
Additional Information
Gene ID 2494
Other Names Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1, NR5A2, B1F, CPF, FTF
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNR5A2 / LRH1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR5A2
Synonyms B1F, CPF, FTF
Function Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
Cellular Location Nucleus.
Tissue Location Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells
Research Areas
Citations (0)

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This antibody is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).


Structural basis of coactivation of liver receptor homolog-1 by ?-catenin. Yumoto F, Nguyen P, Sablin EP, Baxter JD, Webb P, Fletterick RJ. Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):143-8. PMID: 22187462

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$ 325.00
Cat# AF4048a
(40 western blots)
Availability: 7-10 days
Bulk Size
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