NR5A2 / LRH1 Antibody (internal region)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | O00482 |
Other Accession | NP_995582.1, NP_003813.1, 2494, 26424 (mouse), 60349 (rat) |
Reactivity | Human |
Predicted | Mouse, Rat, Pig, Dog, Cow |
Host | Goat |
Clonality | Polyclonal |
Concentration | 0.5 mg/ml |
Isotype | IgG |
Calculated MW | 61331 Da |
Gene ID | 2494 |
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Other Names | Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1, NR5A2, B1F, CPF, FTF |
Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | NR5A2 / LRH1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NR5A2 |
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Synonyms | B1F, CPF, FTF |
Function | Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti- inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed:20159957). May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38 (By similarity). |
Cellular Location | Nucleus. |
Tissue Location | Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).
References
Structural basis of coactivation of liver receptor homolog-1 by ?-catenin. Yumoto F, Nguyen P, Sablin EP, Baxter JD, Webb P, Fletterick RJ. Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):143-8. PMID: 22187462
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