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Goat Anti-COL4A4 Antibody (internal region (near C terminus))

Purified Goat Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P53420
Other Accession NP_000083.3
Predicted Human
Host Goat
Clonality Polyclonal
Concentration 0.5
Calculated MW 164038 Da
Additional Information
Gene ID 1286
Other Names COL4A4; collagen, type IV, alpha 4; CA44; Collagen IV, alpha-4 polypeptide; collagen alpha-4(IV) chain; collagen of basement membrane, alpha-4 chain
Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen Peptide with sequence C-SEEAIRPYVSR, from the internal region (near C terminus) of the protein sequence according to NP_000083.3.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-COL4A4 Antibody (internal region (near C terminus)) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COL4A4
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Cellular Location Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000255|PROSITE-ProRule:PRU00736} Note=Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina (BL).
Tissue Location Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. Highest levels of expression of alpha 4 type IV collagen are detected in kidney, calvaria, neuroretina and cardiac muscle Lower levels of expression are observed in brain, lung and thymus, and no expression is detected in choroid plexus, liver, adrenal, pancreas, ileum or skin.
Research Areas
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The collagen binding domain of gelatinase A modulates degradation of collagen IV by gelatinase B. Gioia M, Monaco S, Van Den Steen PE, Sbardella D, Grasso G, Marini S, Overall CM, Opdenakker G, Coletta M. J Mol Biol. 2009 Feb \20;386(2):419-34.

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$ 325.00
Cat# AF4159a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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