|Application ||WB, E|
|Other Accession||72145(mouse), 305164(rat), NP_055806.2|
|Predicted||Human, Mouse, Rat, Cow|
|Calculated MW||395258 Da|
|Other Names||WDFY3; WD repeat and FYVE domain containing 3; ALFY; ZFYVE25; WD repeat and FYVE domain-containing protein 3; autophagy-linked FYVE protein|
|Format||Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.|
|Immunogen||Peptide with sequence C-SPERSTRTQQKEFQT, from the internal region of the protein sequence according to NP_055806.2.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-Alfy / WDFY3 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation.|
|Cellular Location||Nucleus membrane. Cytoplasm. Nucleus, PML body. Membrane; Peripheral membrane protein; Cytoplasmic side Note=Colocalizes with autophagic structures in starved cells Shuttles between nucleus and cytoplasm; translocation from the nucleus to cytoplasmic polyubiquitin-containing bodies upon cellular stress is dependent on SQSTM1|
|Tissue Location||Ubiquitous. Expressed in osteoclast and their mononuclear precursors (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
Alfy-dependent elimination of aggregated proteins by macroautophagy: can there be too much of a good thing? Yamamoto A, Simonsen A. Autophagy 2011 Mar 7 (3): 346-50.
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