|Application ||IHC-P, IF, FC|
|Other Accession||5788, 654514|
|Isotype||Mouse / IgG2a, kappa|
|Other Names||Receptor-type tyrosine-protein phosphatase C, 184.108.40.206, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45|
|Format||200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD45RO (T-Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts|
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Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.2. Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138.3. Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315.4. Yamada M, et al. 1996. Stroke 27:1155. 5. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430
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