Neurofilament, phospho (NF-H) (Neuronal Marker) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone NE14 ]
|Application ||IHC, FC|
|Other Accession||4744, 198760|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Chicken, Cow, Guinea Pig, Gerbil, Cat|
|Isotype||Mouse / IgG1|
|Other Names||Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, NEFH, KIAA0845, NFH|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Neurofilament, phospho (NF-H) (Neuronal Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Debus E., et al. Differentiation 25(2), 193-203 (1983). | Gotow T., et al. J. Neuroscience Res. 37(6), 691-713 (1994). | Berglund A.M., et al. J Comp Neurol. 306(3), 393-408 (1991). | Guadano-Ferraz A., , et al. Develop. Brain Res. GOTOBUTTON BM_4_ 56(2), 244-256, (1990). | Obermann W., et al. Eur. J. Biochem 233, 110-115 (1995). | Marx A., et al. Am. J.Pathol. 148(6), 1839-1850 (1996)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.