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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >    Nuclear Mitotic Apparatus Protein (NuMA) Antibody - With BSA and Azide   

Nuclear Mitotic Apparatus Protein (NuMA) Antibody - With BSA and Azide

Mouse Monoclonal Antibody [Clone SPM300 ]

  • IHC -  Nuclear Mitotic Apparatus Protein (NuMA) Antibody - With BSA and Azide AH12021-20
    Formalin-fixed, paraffin-embedded human Tonsil stained with NuMA Monoclonal Antibody (SPM300)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14980
Other Accession 4926, 325978
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgM, kappa
Clone Names SPM300
Calculated MW 228kDa
Additional Information
Gene ID 4926
Other Names Nuclear mitotic apparatus protein 1 {ECO:0000312|HGNC:HGNC:8059}, NuMA protein, Nuclear matrix protein-22, NMP-22, SP-H antigen, NUMA1 (HGNC:8059)
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Nuclear Mitotic Apparatus Protein (NuMA) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NUMA1 (HGNC:8059)
Function Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume (PubMed:10075938). Required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes (PubMed:7769006, PubMed:11956313, PubMed:26195665). Also required for proper alignment of the mitotic spindle during asymmetric cell divisions (PubMed:21816348).
Cellular Location Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Resides in the nuclear matrix during interphase. Dissociates from condensing chromosomes during early prophase and relocates to the spindle poles via dynein/dynamin association; it remains there until the anaphase onset. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. CCSAP is essential for its centrosomal localization (PubMed:26562023). Isoform Numa-s: Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle pole. Note=During interphase, mainly clustered at the centrosomal region in the cytosol. After entry into mitosis, detected at mitotic spindle poles
Research Areas
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Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.


Butschak G et al. New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells. Acta Histochem 1995, 97(1):19-31 | Compton DA and Cleveland DW. NuMA is required for the proper completion of mitosis. J Cell Biol 1993, 120(4):947-57 | Price CM and Pettijohn DE. Redistribution of the nuclear mitotic apparatus protein (NuMA) during mitosis and nuclear assembly. Properties of purified NuMA protein. Exp Cell Res 1986, 166:295-311. | Gueth-Hallonet C et al. Cleavage of the nuclear matrix protein NuMA during apoptosis. Exp Cell Res 1997, 233(1):21-24. |

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Cat# AH12021-20
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