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Anti-Cytokeratin, Basic (Type II or HMW) Antibody
Recombinant Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, FC |
|---|---|
| Primary Accession | Q01546 |
| Other Accession | 654392 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit / IgG, kappa |
| Clone Names | KRTH/1576R |
| Calculated MW | 65841 Da |
| Gene ID | 51350 |
|---|---|
| Other Names | KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9 |
| Application Note | Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml f); ,Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-Cytokeratin, Basic (Type II or HMW) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KRT76 |
|---|---|
| Synonyms | KRT2B, KRT2P |
| Function | Probably contributes to terminal cornification. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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