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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   RAD17 antibody - C-terminal region   

RAD17 antibody - C-terminal region

Rabbit Polyclonal Antibody

     
  • WB - RAD17 antibody - C-terminal region AI10008
    RAD17 antibody - C-terminal region (AI10008) in Hela, HEK293T, Xenopus laevis egg extract, mouse embryonic cells using Western Blot
    WB Suggested Anti-RAD17 Antibody
    Positive Control: Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysate
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Anti-rabbit-HRP
    Secondry Antibody Dilution : 1:3000
    Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS
  • WB - RAD17 antibody - C-terminal region AI10008
    RAD17 antibody - C-terminal region (AI10008) in Human Jurkat cells using Western Blot
    WB Suggested Anti-RAD17 Antibody Titration: 1.25μg/ml
    ELISA Titer: 1:62500
    Positive Control: Jurkat cell lysate
    RAD17 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O75943
Other Accession O75943-4, NP_579919, NM_133341
Reactivity Human, Mouse, Rat, Pig, Dog, Bovine
Predicted Human, Mouse, Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 66 kDa
Additional Information
Gene ID 5884
Alias Symbol CCYC, HRAD17, R24L, RAD17Sp, Rad24, RAD24, RAD17SP
Other Names Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, RAD17, R24L
Target/Specificity RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 100 ul of distilled water. Final anti-RAD17 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
PrecautionsRAD17 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD17
Synonyms R24L
Function Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9- HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Cellular Location Nucleus. Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage
Tissue Location Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
Research Areas
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Background

This is a rabbit polyclonal antibody against RAD17. It was validated on Western Blot using a cell lysate as a positive control. Abgent strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (sales@abgent.com).

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$ 232.00
Cat# AI10008
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