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TSFM antibody - middle region

Rabbit Polyclonal Antibody

     
  • WB - TSFM antibody - middle region AI10017
    TSFM antibody - middle region (AI10017) in Human 293T cells using Western Blot
    Host:Rabbit
    Target Name:TSFM
    Sample Tissue:Human 293T
    Antibody Dilution: 1.0μg/ml
    There is BioGPS gene expression data showing that TSFM is expressed in HEK293T
  • WB - TSFM antibody - middle region AI10017
    TSFM antibody - middle region (AI10017) in Human 721_B cells using Western Blot
    Host:Rabbit
    Target Name:TSFM
    Sample Tissue:Human 721_B
    Antibody Dilution: 1.0μg/ml
    TSFM is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
  • WB - TSFM antibody - middle region AI10017
    TSFM antibody - middle region (AI10017) in Human MCF7 cells using Western Blot
    Host:Rabbit
    Target Name:TSFM
    Sample Tissue:Human MCF7
    Antibody Dilution: 1.0μg/ml
    TSFM is strongly supported by BioGPS gene expression data to be expressed in MCF7
  • WB - TSFM antibody - middle region AI10017
    TSFM antibody - middle region (AI10017) in Human HeLa cells using Western Blot
    WB Suggested Anti-TSFM Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:312500
    Positive Control: Hela cell lysate
    .TSFM is strongly supported by BioGPS gene expression data to be expressed in HeLa
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P43897
Other Accession P43897, NP_005717, NM_005726
Reactivity Human, Mouse, Rat, Rabbit, Guinea Pig, Bovine
Predicted Human, Mouse, Rabbit, Guinea Pig
Host Rabbit
Clonality Polyclonal
Calculated MW 38 kDa
Additional Information
Gene ID 10102
Alias Symbol COXPD3, EF-TS, EF-Tsmt, EFTS, EFTSMT
Other Names Elongation factor Ts, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03135}, EF-Ts {ECO:0000255|HAMAP-Rule:MF_03135}, EF-TsMt {ECO:0000255|HAMAP-Rule:MF_03135}, TSFM {ECO:0000255|HAMAP-Rule:MF_03135}
Target/Specificity The TSFM protein is a mitochondrial translation elongation factor. Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes.Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).[supplied by OMIM].
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-TSFM antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
PrecautionsTSFM antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSFM {ECO:0000255|HAMAP-Rule:MF_03135}
Function Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
Cellular Location Mitochondrion.
Tissue Location Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney
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Background

This is a rabbit polyclonal antibody against TSFM. It was validated on Western Blot using a cell lysate as a positive control. Abgent strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (sales@abgent.com).

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$ 293.00
Cat# AI10017
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