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SIAH1 antibody - N-terminal region

Rabbit Polyclonal Antibody

  • IHC - SIAH1 antibody - N-terminal region AI10114
    SIAH1 antibody (AI10114) in Human Heart cells using Immunohistochemistry
    Rabbit Anti-SIAH1 antibody
    Catalog Number: AI10114
    Paraffin Embedded Tissue: Human Heart cell
    Cellular Data: Epithelial cells of renal tubule
    Antibody Concentration: 4.0-8.0 μg/ml
    Magnification: 400X
  • WB - SIAH1 antibody - N-terminal region AI10114
    SIAH1 antibody - N-terminal region (AI10114) in Human HEK293T cells using Western Blot
    Application: Western blotting
    Species+tissue/cell type: Human embryonic kidney 293T
    How many ug’s of tissue/cell lysate
    run on the gel:1: 50 ug human HEK-293T cell lysate

    Primary Antibody Dilution: 1:1000
    Secondary Antibody: Anti-rabbit-IgG
    Secondary Antibody Dilution: 1:5000
    SIAH1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
  • WB - SIAH1 antibody - N-terminal region AI10114
    SIAH1 antibody - N-terminal region (AI10114) in Human Jurkat cells using Western Blot
    WB Suggested Anti-SIAH1 Antibody Titration: 1.25μg/ml
    ELISA Titer: 1:312500
    Positive Control: Jurkat cell lysate
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8IUQ4
Other Accession Q8IUQ4-2, NP_001006611, NM_001006610
Reactivity Human, Mouse, Rat, Rabbit, Zebrafish, Dog, Guinea Pig, Horse, Bovine
Predicted Human, Mouse, Rat, Zebrafish, Chicken, Dog, Guinea Pig, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 35 kDa
Additional Information
Gene ID 6477
Alias Symbol SIAH1A
Other Names E3 ubiquitin-protein ligase SIAH1, 632-, Seven in absentia homolog 1, Siah-1, Siah-1a, SIAH1, HUMSIAH
Target/Specificity SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 100 ul of distilled water. Final anti-SIAH1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
PrecautionsSIAH1 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIAH1
Synonyms HUMSIAH
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S- nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear
Tissue Location Widely expressed at a low level. Down- regulated in advanced hepatocellular carcinomas
Research Areas
Citations (0)

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This is a rabbit polyclonal antibody against SIAH1. It was validated on Western Blot using a cell lysate as a positive control. Abgent strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (

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$ 232.00
Cat# AI10114
(40 western blots)
Availability: 2-3 days
Bulk Size
Seasonal Special on Bulk Order
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