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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   YBX1 antibody - middle region   

YBX1 antibody - middle region

Rabbit Polyclonal Antibody

     
  • IHC - YBX1 antibody - middle region AI10130
    YBX1 antibody - middle region (AI10130) in Human Placenta cells using Immunohistochemistry
    Placenta
  • WB - YBX1 antibody - middle region AI10130
    YBX1 antibody - middle region (AI10130) in mouse brain cells using Western Blot
    WB Suggested Anti-YBX1 Antibody
    Positive Control: Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus
    Primary Antibody Dilution : 1:1000
    Secondary Antibody : Anti rabbit - IR-dye
    Secondry Antibody Dilution : 1:10,000
    Submitted by: Anonymous
  • WB - YBX1 antibody - middle region AI10130
    YBX1 antibody - middle region (AI10130) in Human Spleen cells using Western Blot
    WB Suggested Anti-YBX1 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:312500
    Positive Control: Human Spleen
  • WB - YBX1 antibody - middle region AI10130
    YBX1 antibody - middle region (AI10130) in Human NT-2 cells using Western Blot
    YBX1 antibody - middle region (AI10130) validated by WB using 1. Human NT-2 cells (60ug) at 2μg/ml.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P67809
Other Accession P67809, NP_004550, NM_004559
Reactivity Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Horse, Sheep, Bovine
Predicted Human, Mouse, Rat, Pig, Chicken, Dog, Sheep, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 36 kDa
Additional Information
Gene ID 4904
Alias Symbol BP-8, CSDA2, CSDB, DBPB, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP-1, NSEP1, YB-1, YB1
Other Names Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, DBPB, Enhancer factor I subunit A, EFI-A, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1, NSEP1, YB1
Target/Specificity YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. YBX1 binds and stabilizes cytoplasmic mRNA. YBX1 contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. YBX1 binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. YBX1 regulates the transcription of numerous genes. YBX1 promotes separation of DNA strands that contain mismatches or are modified by cisplatin. YBX1 has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). YBX1 may play a role in DNA repair. YBX1 is the component of the CRD-mediated complex that promotes MYC mRNA stability.
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-YBX1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
PrecautionsYBX1 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name YBX1
Synonyms NSEP1, YB1
Function Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro.
Cellular Location Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress
Research Areas
Citations (0)

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Background

This is a rabbit polyclonal antibody against YBX1. It was validated on Western Blot using a cell lysate as a positive control. Abgent strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (sales@abgent.com).

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$ 293.00
Cat# AI10130
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