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FOXA1 antibody - C-terminal region

Rabbit Polyclonal Antibody

     
  • WB - FOXA1 antibody - C-terminal region AI10392
    WB Suggested Anti-FOXA1 Antibody Titration: .2-1 μg/ml
    Positive Control: Transfected 293T
    detail
  • WB - FOXA1 antibody - C-terminal region AI10392
    WB Suggested Anti-FOXA1 Antibody
    Positive Control: Lane 1: 2ug MCF7 cell lysate
    s Lane 2: 2ug MCF7 cell lysate
    s Lane 3: 2ug MCF7 cell lysate
    s Lane 4: 2ug MCF7 cell lysate
    s Lane 5: 2ug MCF7 with FoxA1 knockdown Lane 6: 2ug MCF7 with FoxA1 knockdown

    Primary Antibody Dilution :
    1:1

    Secondary Antibody :
    Anti-rabbit-HRP

    Secondry Antibody Dilution :
    1:2

    Submitted by:
    Fu, Xiaoyong, Baylor College of Medicine

    FOXA1 is supported by BioGPS gene expression data to be expressed in MCF7

    detail
  • WB - FOXA1 antibody - C-terminal region AI10392
    WB Suggested Anti-FOXA1 antibody Titration: 1 μg/ml

    Sample Type: Human heart
    detail
  • WB - FOXA1 antibody - C-terminal region AI10392
    WB Suggested Anti-FOXA1 antibody Titration: 1 μg/ml

    Sample Type: Human liver
    detail
  • IHC - FOXA1 antibody - C-terminal region AI10392
    Rabbit Anti-FOXA1 antibody

    Catalog Number: AI1392

    Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
    Observed Staining: Nuclei in adipocytes but not in cardiomyocytes

    Primary Antibody
    Concentration: 1:1

    Secondary Antibody: Donkey anti-Rabbit-Cy2/3

    Secondary Antibody
    Concentration: 1:2

    Magnification: 2X

    Exposure Time: .5 – 2. sec

    Protocol located in Reviews and Data.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q0JTB6
Other Accession NM_004496, NP_004487
Reactivity Human, Mouse, Rat, Zebrafish, Pig, Yeast, Bovine, Dog
Predicted Human, Mouse, Rat, Zebrafish, Pig, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 49kDa
Additional Information
Alias Symbol HNF3A, TCF3A
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-FOXA1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsFOXA1 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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References

Williamson,E.A., (2006) Oncogene 25 (9), 1391-1399Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

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$ 389.00
Cat# AI10392
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