|Application ||WB, IHC|
|Other Accession||NM_006756, NP_006747|
|Reactivity||Human, Mouse, Rat, Horse, Dog|
|Predicted||Human, Mouse, Rat, Pig, Chicken, Guinea Pig, Dog|
|Alias Symbol||SII, TCEA, TF2S, GTF2S, TFIIS|
|Other Names||Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIIS.o, TCEA1, GTF2S, TFIIS|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-TCEA1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||TCEA1 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.|
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Provided below are standard protocols that you may find useful for product applications.
Kimura,K., (2006) Genome Res. 16 (1), 55-65Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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