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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CHST4 antibody - middle region   

CHST4 antibody - middle region

Rabbit Polyclonal Antibody

  • WB - CHST4 antibody - middle region AI10880

    WB Suggested Anti-CHST4 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:312500
    Positive Control: HepG2 cell lysate
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NCG5
Other Accession NM_005769, NP_005760
Reactivity Human, Rabbit, Horse, Bovine, Dog
Predicted Human, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 45kDa
Additional Information
Gene ID 10164
Other Names Carbohydrate sulfotransferase 4, 2.8.2.-, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3, GST-3, High endothelial cells N-acetylglucosamine 6-O-sulfotransferase, HEC-GlcNAc6ST, L-selectin ligand sulfotransferase, LSST, N-acetylglucosamine 6-O-sulfotransferase 2, GlcNAc6ST-2, Gn6st-2, CHST4
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-CHST4 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsCHST4 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHST4
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2- branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1- 3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma.
Research Areas
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Akama,T.O., et al., (2002) J. Biol. Chem. 277 (45), 42505-42513Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

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$ 293.00
Cat# AI10880
(40 western blots)
Availability: 2-3 days
Bulk Size
Seasonal Special on Bulk Order
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