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> home > Products > Primary Antibodies > Antibody Collections > Zebrafish > ANKZF1 antibody - N-terminal region
ANKZF1 antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q9H8Y5 |
| Other Accession | NM_018089, NP_060559 |
| Reactivity | Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Horse, Bovine, Dog |
| Predicted | Human, Rat, Pig, Horse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 81kDa |
| Gene ID | 55139 |
|---|---|
| Alias Symbol | FLJ10415, FLJ13144, ZNF744 |
| Other Names | Ankyrin repeat and zinc finger domain-containing protein 1, Zinc finger protein 744, ANKZF1, ZNF744 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-ANKZF1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | ANKZF1 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ANKZF1 {ECO:0000303|PubMed:21896481, ECO:0000312|HGNC:HGNC:25527} |
|---|---|
| Function | Endonuclease that cleaves polypeptidyl-tRNAs downstream of the ribosome-associated quality control (RQC) pathway to release incompletely synthesized polypeptides for degradation (PubMed:30244831, PubMed:29632312, PubMed:31011209). The RQC pathway disassembles aberrantly stalled translation complexes to recycle or degrade the constituent parts (PubMed:30244831, PubMed:29632312, PubMed:31011209). ANKZF1 acts downstream disassembly of stalled ribosomes and specifically cleaves off the terminal 3'-CCA nucleotides universal to all tRNAs from polypeptidyl-tRNAs, releasing (1) ubiquitinated polypeptides from 60S ribosomal subunit for degradation and (2) cleaved tRNAs (PubMed:31011209). ANKZF1-cleaved tRNAs are then repaired and recycled by ELAC1 and TRNT1 (PubMed:31011209, PubMed:32075755). Also plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress (PubMed:28302725). |
| Cellular Location | Cytoplasm. Note=Translocates to the mitochondria upon exposure to hydrogen peroxide. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Olsen,J.V., (2006) Cell 127 (3), 635-648 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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$ 389.00
Cat# AI10914
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