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> home > Products > Primary Antibodies > Antibody Collections > Zebrafish > TIMELESS antibody - N-terminal region
TIMELESS antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
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- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q9UNS1 |
| Other Accession | NM_003920, NP_003911 |
| Reactivity | Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Sheep, Horse, Bovine, Dog |
| Predicted | Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 139kDa |
| Gene ID | 8914 |
|---|---|
| Alias Symbol | TIM, TIM1, hTIM |
| Other Names | Protein timeless homolog, hTIM, TIMELESS {ECO:0000312|EMBL:AAH50557.1} |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 100 ul of distilled water. Final anti-TIMELESS antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | TIMELESS antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TIMELESS {ECO:0000312|EMBL:AAH50557.1} |
|---|---|
| Function | Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication, DNA repair and in the regulation of the circadian clock (PubMed:9856465, PubMed:17141802, PubMed:17296725, PubMed:23418588, PubMed:26344098, PubMed:23359676, PubMed:35585232, PubMed:31138685, PubMed:32705708). Required to stabilize replication forks during DNA replication by forming a complex with TIPIN: this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PubMed:17141802, PubMed:17296725, PubMed:23359676, PubMed:35585232). During DNA replication, inhibits the CMG complex ATPase activity and activates DNA polymerases catalytic activities, coupling DNA unwinding and DNA synthesis (PubMed:23359676). TIMELESS promotes TIPIN nuclear localization (PubMed:17141802, PubMed:17296725). Plays a role in maintaining processive DNA replication past genomic guanine-rich DNA sequences that form G- quadruplex (G4) structures, possibly together with DDX1 (PubMed:32705708). Involved in cell survival after DNA damage or replication stress by promoting DNA repair (PubMed:17141802, PubMed:17296725, PubMed:26344098, PubMed:30356214). In response to double-strand breaks (DSBs), accumulates at DNA damage sites and promotes homologous recombination repair via its interaction with PARP1 (PubMed:26344098, PubMed:30356214, PubMed:31138685). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PubMed:15798197). Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock (PubMed:23418588, PubMed:31138685). Negatively regulates CLOCK|NPAS2- ARTNL/BMAL1|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus (PubMed:9856465, PubMed:31138685). May play a role as destabilizer of the PER2-CRY2 complex (PubMed:31138685). May also play an important role in epithelial cell morphogenesis and formation of branching tubules (By similarity). |
| Cellular Location | Nucleus. Chromosome Note=In response to double-strand breaks (DSBs), accumulates at DNA damage sites via its interaction with PARP1 |
| Tissue Location | Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Murre,C., (2005) Mol. Cell. Biol. 25 (8), 3109-3116Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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$ 339.00
Cat# AI11409
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