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IKZF4 antibody - N-terminal region

Rabbit Polyclonal Antibody

     
  • IHC - IKZF4 antibody - N-terminal region AI11576
    Rabbit Anti-IKZF4 Antibody
    Catalog Number: AI11576
    Formalin Fixed Paraffin Embedded Tissue: Human Adult heart Observed Staining: Nuclear
    Primary Antibody
    Concentration: 1:100
    Secondary Antibody: Donkey anti-Rabbit-Cy2/3
    Secondary Antibody
    Concentration: 1:200
    Magnification: 20X
    Exposure Time: 0.5 – 2.0 sec
    Protocol located in Reviews and Data.
    detail
  • WB - IKZF4 antibody - N-terminal region AI11576

    WB Suggested Anti-IKZF4 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:62500
    Positive Control: 721_B cell lysate
    IKZF4 is supported by BioGPS gene expression data to be expressed in 721_B
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9H2S9
Other Accession NM_022465, NP_071910
Reactivity Human, Mouse, Pig
Predicted Human, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 64kDa
Additional Information
Gene ID 64375
Alias Symbol EOS, KIAA1782, ZNFN1A4
Other Names Zinc finger protein Eos, Ikaros family zinc finger protein 4, IKZF4, KIAA1782, ZNFN1A4
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-IKZF4 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsIKZF4 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IKZF4
Synonyms KIAA1782, ZNFN1A4
Function DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros- binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3- mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1 (By similarity).
Cellular Location Nucleus.
Tissue Location Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K-562. Highly expressed in THP-1 and M-07e cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively
Citations (0)
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References

Perdomo,J. (2002) Eur. J. Biochem. 269 (23), 5885-5892 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

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$ 389.00
Cat# AI11576
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