|Application ||WB, IHC|
|Other Accession||NM_016042, NP_057126|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Dog|
|Predicted||Human, Mouse, Pig, Chicken|
|Alias Symbol||p10, RRP40, Rrp40p, CGI-102, hRrp-40, hRrp40p, bA3J10.7, RP11-3J10.8|
|Other Names||Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, p10, EXOSC3, RRP40|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-EXOSC3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||EXOSC3 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5.|
|Cellular Location||Cytoplasm. Nucleus, nucleolus. Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
Lehner,B. (2004) Genome Res. 14 (7), 1315-1323Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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