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POLRMT Antibody - C-terminal region

Rabbit Polyclonal Antibody

     
  • WB - POLRMT Antibody - C-terminal region AI12927

    Host: Rabbit
    Target Name: POLRMT
    Sample Tissue: OVCAR-3 Whole cell lysate
    s
    Antibody Dilution: 1.0μg/mlPOLRMT is supported by BioGPS gene expression data to be expressed in OVCAR3
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O00411
Other Accession NM_005035, NP_005026
Reactivity Human, Mouse, Rat, Yeast, Bovine, Guinea Pig, Dog
Predicted Human, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 135kDa
Additional Information
Gene ID 5442
Alias Symbol APOLMT, MTRNAP, MTRPOL, h-mtRPOL
Other Names DNA-directed RNA polymerase, mitochondrial, MtRPOL, 2.7.7.6, POLRMT
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-POLRMT antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsPOLRMT Antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLRMT (HGNC:9200)
Function DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:21278163, PubMed:33602924). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non- template strand (PubMed:29149603). Has DNA primase activity (PubMed:18685103, PubMed:33602924). Catalyzes the synthesis of short RNA primers that are necessary for the initiation of lagging-strand DNA synthesis from the origin of light-strand DNA replication (OriL) (PubMed:18685103, PubMed:33602924).
Cellular Location Mitochondrion
Research Areas
Citations (0)
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$ 389.00
Cat# AI12927
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