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Bckdk antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q00972 |
| Other Accession | NM_019244, NP_062117 |
| Reactivity | Human, Mouse, Rat, Rabbit, Bovine, Guinea Pig, Dog |
| Predicted | Human, Mouse, Pig, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 45kDa |
| Gene ID | 29603 |
|---|---|
| Other Names | [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, 2.7.11.4, Branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDHKIN, Bckdk |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-Bckdk antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | Bckdk antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Bckdk |
|---|---|
| Function | Serine/threonine-protein kinase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched- chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle (PubMed:1377677, PubMed:7649998) (Probable). Phosphorylates and inactivates mitochondrial BCKDH complex a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Associates with the E2 component of BCKDH complex and phosphorylates BCKDHA on Ser-333, leading to conformational changes that interrupt substrate channeling between E1 and E2 and inactivates the BCKDH complex (PubMed:1377677, PubMed:7649998) (Probable). phosphorylates ACLY on Ser-455 in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and glucogenesis, respectively (Probable). Recognizes phosphosites having SxxE/D canonical motif (By similarity). |
| Cellular Location | Mitochondrion matrix. Mitochondrion. Note=Detected in the cytosolic compartment of liver cells. |
| Tissue Location | Expressed in heart and liver. |
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Popov K.M.,et al.J. Biol. Chem. 267:13127-13130(1992).
Harris R.A.,et al.Adv. Enzyme Regul. 32:267-284(1992).
Davie J.R.,et al.J. Biol. Chem. 270:19861-19867(1995).
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$ 389.00
Cat# AI13374
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