|Other Accession||NM_001040158, NP_001035248|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Guinea Pig, Dog|
|Predicted||Mouse, Rat, Pig, Chicken, Horse, Bovine, Dog|
|Alias Symbol||H2A.y, H2A/y, H2AF12M, H2AFJ, MACROH2A1.1, mH2A1, macroH2A1.2|
|Other Names||Core histone macro-H2A.1, Histone macroH2A1, mH2A1, Histone H2A.y, H2A/y, Medulloblastoma antigen MU-MB-50.205, H2AFY, MACROH2A1|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-H2AFY antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||H2AFY antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation.|
|Cellular Location||Nucleus Chromosome Note=Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin|
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Provided below are standard protocols that you may find useful for product applications.
Lee Y.,et al.Biochim. Biophys. Acta 1399:73-77(1998).
Mao M.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Schmutz J.,et al.Nature 431:268-274(2004).
Behrends U.,et al.Int. J. Cancer 106:244-251(2003).
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