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>   home   >   Products   >   Primary Antibodies   >   POLD3 antibody - C-terminal region   

POLD3 antibody - C-terminal region

Rabbit Polyclonal Antibody

     
  • WB - POLD3 antibody - C-terminal region AI14772

    WB Suggested Anti-POLD3 Antibody Titration: 1.0 μg/ml
    Positive Control: Jurkat Whole CellPOLD3 is supported by BioGPS gene expression data to be expressed in Jurkat
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q15054
Other Accession NM_006591, NP_006582
Reactivity Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Rabbit, Chicken, Horse, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 51kDa
Additional Information
Gene ID 10714
Alias Symbol KIAA0039, MGC119642, MGC119643, P66, P68
Other Names DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, POLD3, KIAA0039
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-POLD3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsPOLD3 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLD3
Synonyms KIAA0039
Function As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA (PubMed:10219083, PubMed:10852724, PubMed:11595739, PubMed:16510448, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol- delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion (PubMed:19074196, PubMed:25628356, PubMed:27185888). Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q9EQ28}. Nucleus. Note=Partially colocalizes with PCNA and POLD1 at S phase replication sites (PubMed:11595739) Recruited to DNA damage sites within 2 hours following UV irradiation (PubMed:20227374, PubMed:22801543)
Citations (0)

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References

Nomura N.,et al.DNA Res. 1:27-35(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.

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$ 293.00
Cat# AI14772
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