|Application ||WB, IHC|
|Other Accession||NM_053463.1, NP_445915|
|Reactivity||Mouse, Rat, Dog|
|Predicted||Mouse, Rat, Dog|
|Other Names||Nucleobindin-1, Bone 63 kDa calcium-binding protein, CALNUC, Nucb1, Nuc|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 100 ul of distilled water. Final anti-NUCB1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||NUCB1 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis.|
|Cellular Location||Golgi apparatus, cis-Golgi network membrane; Peripheral membrane protein; Lumenal side. Cytoplasm Secreted. Note=A small fraction of the protein may be cytoplasmic. In bone at least part of it is found in the extracellular matrix and in the culture medium of calvaria explants.|
|Tissue Location||Localized in the soft tissue in the center of the osteon and in the osteocyte lacuna. Minor constituent of the mineralized matrix of bone. Detected in calvaria, rib cartilage, liver, kidney, spleen, brain, lung, skeletal and heart muscle|
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Provided below are standard protocols that you may find useful for product applications.
Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
Wendel M.,et al.J. Biol. Chem. 270:6125-6133(1995).
Long X.,et al.Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
Adams L.A.,et al.Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
Lin P.,et al.J. Cell Biol. 141:1515-1527(1998).
Lin P.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:674-679(2000).
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