|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:10000-1:40000), IHC-P (10-20 µg/ml), WB (1:500-1:3000)|
|Other Names||Huntingtin, Huntington disease protein, HD protein, HTT, HD, IT15|
|Target/Specificity||Amino acids 416 to 424 of Human huntingtin protein phosphorylated at Ser421|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||HTT / Huntingtin Antibody (phospho-Ser421) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in microtubule-mediated transport or vesicle function.|
|Cellular Location||Cytoplasm. Nucleus. Note=The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington disease neurons. Shuttles between cytoplasm and nucleus in a Ran GTPase-independent manner|
|Tissue Location||Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.|
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Provided below are standard protocols that you may find useful for product applications.
May play a role in microtubule-mediated transport or vesicle function.
Macdonald M.,et al.Cell 72:971-983(1993).
Matsuyama N.,et al.Genomics 69:72-85(2000).
Hillier L.W.,et al.Nature 434:724-731(2005).
Ambrose C.M.,et al.Somat. Cell Mol. Genet. 20:27-38(1994).
Lin B.,et al.Genomics 25:707-715(1995).
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