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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   GLI3 Antibody (aa41-57)   

GLI3 Antibody (aa41-57)

Rabbit Polyclonal Antibody

     
  • IHC - GLI3 Antibody (aa41-57) ALS11238
    Anti-GLI3 antibody IHC of human brain-glioblastoma.
  • IF - GLI3 Antibody (aa41-57) ALS11238
    Anti-Gli-3 Antibody - Immunofluorescence.
  • IF - GLI3 Antibody (aa41-57) ALS11238
    Anti-Gli-3 Antibody - Immunofluorescence.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E
Primary Accession P10071
Reactivity Human, Chicken, Quail, Xenopus, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 170kDa
Dilution ELISA (1:6000-1:30000), IHC-P (1.25 µg/ml), WB (1:500-1:2000)
Additional Information
Gene ID 2737
Other Names Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3-190, GLI3 full length protein, GLI3FL, Transcriptional repressor GLI3R, GLI3 C-terminally truncated form, GLI3 form of 83 kDa, GLI3-83, GLI3
Target/Specificity Amino acids 41-57 of human Gli-3 protein.
Reconstitution & Storage Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
PrecautionsGLI3 Antibody (aa41-57) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLI3
Function Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Cellular Location Nucleus. Cytoplasm. Cell projection, cilium. Note=GLI3FL is localized predominantly in the cytoplasm while GLI3R resides mainly in the nucleus. Ciliary accumulation requires the presence of KIF7 and SMO. Translocation to the nucleus is promoted by interaction with ZIC1
Tissue Location Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium
Research Areas
Citations (0)

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Background

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.

References

Ruppert J.M.,et al.Mol. Cell. Biol. 10:5408-5415(1990).
Kalff-Suske M.,et al.Hum. Mol. Genet. 8:1769-1777(1999).
Hillier L.W.,et al.Nature 424:157-164(2003).
Scherer S.W.,et al.Science 300:767-772(2003).
Ruppert J.M.,et al.Mol. Cell. Biol. 8:3104-3113(1988).

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$ 395.00
Cat# ALS11238
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