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DAPK3 / ZIP Kinase Antibody (Internal)

Rabbit Polyclonal Antibody

     
  • ICC - DAPK3 / ZIP Kinase Antibody (Internal) ALS11448
    Immunocytochemistry of ZIP kinase in Jurkat cells with ZIP kinase antibody at 10 ug/ml.
  • IF - DAPK3 / ZIP Kinase Antibody (Internal) ALS11448
    Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 ug/ml.
  • WB - DAPK3 / ZIP Kinase Antibody (Internal) ALS11448
    Western blot of ZIP kinase in HeLa (H) and Jurkat (J) whole cell lysates with ZIP kinase...
  • IHC - DAPK3 / ZIP Kinase Antibody (Internal) ALS11448
    Anti-DAPK3 antibody IHC of human brain, cortex.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession O43293
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 53kDa
Dilution IHC-P (2.5 µg/ml), WB (1:500-1:1000),
Additional Information
Gene ID 1613
Other Names Death-associated protein kinase 3, DAP kinase 3, 2.7.11.1, DAP-like kinase, Dlk, MYPT1 kinase, Zipper-interacting protein kinase, ZIP-kinase, DAPK3, ZIPK
Target/Specificity peptide corresponding to amino acids near the center of human ZIP kinase
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsDAPK3 / ZIP Kinase Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DAPK3
Synonyms ZIPK
Function Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase- dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.
Cellular Location Nucleus Cytoplasm. Note=Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. Isoform 2: Nucleus. Cytoplasm
Tissue Location Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle
Research Areas
Citations (0)

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Background

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase- dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

References

Kawai T.,et al.Mol. Cell. Biol. 18:1642-1651(1998).
Murata-Hori M.,et al.FEBS Lett. 451:81-84(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Takamoto N.,et al.Arch. Biochem. Biophys. 456:194-203(2006).
Niiro N.,et al.J. Biol. Chem. 276:29567-29574(2001).

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$ 395.00
Cat# ALS11448
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