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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   HUWE1 / ARFBP1 Antibody (C-Terminus)   

HUWE1 / ARFBP1 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • IHC - HUWE1 / ARFBP1 Antibody (C-Terminus) ALS11687
    Anti-HUWE1 antibody IHC of human brain, cortex.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q7Z6Z7
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 482kDa
Dilution IHC-P (5 µg/ml), WB (2 µg/ml),
Additional Information
Gene ID 10075
Other Names E3 ubiquitin-protein ligase HUWE1, 6.3.2.-, ARF-binding protein 1, ARF-BP1, HECT, UBA and WWE domain-containing protein 1, Homologous to E6AP carboxyl terminus homologous protein 9, HectH9, Large structure of UREB1, LASU1, Mcl-1 ubiquitin ligase E3, Mule, Upstream regulatory element-binding protein 1, URE-B1, URE-binding protein 1, HUWE1, KIAA0312, KIAA1578, UREB1
Target/Specificity a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsHUWE1 / ARFBP1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HUWE1
Synonyms KIAA0312, KIAA1578, UREB1
Function E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4.
Cellular Location Cytoplasm. Nucleus. Note=Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells. {ECO:0000250|UniProtKB:Q7TMY8}
Tissue Location Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas
Research Areas
Citations (0)

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Background

E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4.

References

Chen D.,et al.Cell 121:1071-1083(2005).
Zhong Q.,et al.Cell 121:1085-1095(2005).
Liu Z.,et al.Mol. Cell. Biol. 25:2819-2831(2005).
Ross M.T.,et al.Nature 434:325-337(2005).
Miyazaki K.,et al.Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.

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$ 395.00
Cat# ALS11687
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