|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat, Zebrafish, Chicken, Opossum, Horse, Dog|
|Dilution||IHC-P (5 µg/ml), WB (0.5-2 µg/ml),|
|Other Names||E3 ubiquitin-protein ligase MARCH4, 6.3.2.-, Membrane-associated RING finger protein 4, Membrane-associated RING-CH protein IV, MARCH-IV, RING finger protein 174, MARCH4, KIAA1399, RNF174|
|Target/Specificity||A portion of amino acids 300-350 of human RNF174|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||RNF174 / MARCH4 Antibody (aa300-350) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.|
|Cellular Location||Golgi apparatus membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in brain and placenta.|
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Provided below are standard protocols that you may find useful for product applications.
E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Nagase T.,et al.DNA Res. 7:65-73(2000).
Bartee E.,et al.J. Virol. 78:1109-1120(2004).
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