|Application ||WB, IHC-P|
|Dilution||IHC-P (5 µg/ml), WB (1-3 µg/ml),|
|Other Names||Structural maintenance of chromosomes protein 4, SMC protein 4, SMC-4, Chromosome-associated polypeptide C, hCAP-C, XCAP-C homolog, SMC4, CAPC, SMC4L1|
|Target/Specificity||A synthetic peptide corresponding to amino acids 281-297 of human CAP-C. Genbank accession no. NP_001002800.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||SMC4 Antibody (aa281-297) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.|
|Cellular Location||Nucleus. Cytoplasm. Chromosome. Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase|
|Tissue Location||Widely expressed. Higher expression in testis, colon, thymus.|
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Provided below are standard protocols that you may find useful for product applications.
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Nishiwaki T.,et al.J. Hum. Genet. 44:197-202(1999).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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