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DCTN1 / Dynactin 1 Antibody (C-Terminus)

Goat Polyclonal Antibody

     
  • WB - DCTN1 / Dynactin 1 Antibody (C-Terminus) ALS12191
    Antibody staining (1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane).
  • IHC - DCTN1 / Dynactin 1 Antibody (C-Terminus) ALS12191
    Anti-DCTN1 antibody IHC of human brain, cerebellum.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q14203
Reactivity Human, Mouse, Hamster, Monkey, Horse
Host Goat
Clonality Polyclonal
Calculated MW 142kDa
Dilution ELISA (1:128000), IHC-P (2.5 µg/ml), WB (1:128000)
Additional Information
Gene ID 1639
Other Names Dynactin subunit 1, 150 kDa dynein-associated polypeptide, DAP-150, DP-150, p135, p150-glued, DCTN1
Target/Specificity Human DCTN1. This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).
Reconstitution & Storage Store at -20°C. Minimize freezing and thawing.
PrecautionsDCTN1 / Dynactin 1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DCTN1
Function Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed:25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed:23874158). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitement to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed:23386061). Plays a role in primary cilia formation (PubMed:25774020).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, spindle. Nucleus envelope. Note=Localizes to microtubule plus ends (PubMed:17828277, PubMed:22777741, PubMed:25774020) Localizes preferentially to the ends of tyrosinated microtubules (PubMed:26972003). Localization at centrosome is regulated by SLK- dependent phosphorylation (PubMed:23985322). Localizes to centrosome in a PARKDA-dependent manner (PubMed:20719959) Localizes to the subdistal appendage region of the centriole in a KIF3A-dependent manner (PubMed:23386061). PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope (PubMed:20679239)
Tissue Location Brain.
Research Areas
Citations (0)

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Background

Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein- dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.

References

Collin G.B.,et al.Genomics 53:359-364(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Holzbaur E.L.F.,et al.Genomics 31:398-399(1996).

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$ 395.00
Cat# ALS12191
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